WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 3939191 Q20 bases: 3846029(97.635%) Q30 bases: 3722600(94.5016%) Read1 after filtering: total reads: 25706 total bases: 2013087 Q20 bases: 1956422(97.1852%) Q30 bases: 1888290(93.8007%) Filtering result: reads passed filter: 25706 reads failed due to low quality: 235 reads failed due to too many N: 0 reads failed due to too short: 24059 reads with adapter trimmed: 713 bases trimmed due to adapters: 5687 reads with polyX in 3' end: 27713 bases trimmed in polyX tail: 1383781 Duplication rate (may be overestimated since this is SE data): 22.838% JSON report: SRX7656988.fastp.json HTML report: SRX7656988.fastp.html fastp --in1 SRX7656988.fastq.gz --out1 SRX7656988.fastp.fastq.gz --thread 6 --json SRX7656988.fastp.json --html SRX7656988.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds