WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4105076 Q20 bases: 4017682(97.8711%) Q30 bases: 3897723(94.9489%) Read1 after filtering: total reads: 23397 total bases: 1861289 Q20 bases: 1811730(97.3374%) Q30 bases: 1751775(94.1162%) Filtering result: reads passed filter: 23397 reads failed due to low quality: 165 reads failed due to too many N: 0 reads failed due to too short: 26438 reads with adapter trimmed: 517 bases trimmed due to adapters: 4133 reads with polyX in 3' end: 29761 bases trimmed in polyX tail: 1708803 Duplication rate (may be overestimated since this is SE data): 29.21% JSON report: SRX7656987.fastp.json HTML report: SRX7656987.fastp.html fastp --in1 SRX7656987.fastq.gz --out1 SRX7656987.fastp.fastq.gz --thread 6 --json SRX7656987.fastp.json --html SRX7656987.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds