WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4459461 Q20 bases: 4365899(97.9019%) Q30 bases: 4238812(95.0521%) Read1 after filtering: total reads: 31814 total bases: 2687114 Q20 bases: 2622757(97.605%) Q30 bases: 2540702(94.5513%) Filtering result: reads passed filter: 31814 reads failed due to low quality: 177 reads failed due to too many N: 0 reads failed due to too short: 18009 reads with adapter trimmed: 344 bases trimmed due to adapters: 2666 reads with polyX in 3' end: 20969 bases trimmed in polyX tail: 1241882 Duplication rate (may be overestimated since this is SE data): 17.336% JSON report: SRX7656986.fastp.json HTML report: SRX7656986.fastp.html fastp --in1 SRX7656986.fastq.gz --out1 SRX7656986.fastp.fastq.gz --thread 6 --json SRX7656986.fastp.json --html SRX7656986.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds