WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4110419 Q20 bases: 4018356(97.7603%) Q30 bases: 3892747(94.7044%) Read1 after filtering: total reads: 22882 total bases: 1819835 Q20 bases: 1769145(97.2146%) Q30 bases: 1707672(93.8366%) Filtering result: reads passed filter: 22882 reads failed due to low quality: 195 reads failed due to too many N: 0 reads failed due to too short: 26923 reads with adapter trimmed: 500 bases trimmed due to adapters: 4023 reads with polyX in 3' end: 29726 bases trimmed in polyX tail: 1747737 Duplication rate (may be overestimated since this is SE data): 25.72% JSON report: SRX7656984.fastp.json HTML report: SRX7656984.fastp.html fastp --in1 SRX7656984.fastq.gz --out1 SRX7656984.fastp.fastq.gz --thread 6 --json SRX7656984.fastp.json --html SRX7656984.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds