WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4624049 Q20 bases: 4527387(97.9096%) Q30 bases: 4395578(95.0591%) Read1 after filtering: total reads: 39029 total bases: 3325848 Q20 bases: 3251552(97.7661%) Q30 bases: 3154943(94.8613%) Filtering result: reads passed filter: 39029 reads failed due to low quality: 114 reads failed due to too many N: 0 reads failed due to too short: 10857 reads with adapter trimmed: 276 bases trimmed due to adapters: 2144 reads with polyX in 3' end: 15337 bases trimmed in polyX tail: 776959 Duplication rate (may be overestimated since this is SE data): 8.124% JSON report: SRX7656983.fastp.json HTML report: SRX7656983.fastp.html fastp --in1 SRX7656983.fastq.gz --out1 SRX7656983.fastp.fastq.gz --thread 6 --json SRX7656983.fastp.json --html SRX7656983.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds