WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4401724 Q20 bases: 4308865(97.8904%) Q30 bases: 4181326(94.9929%) Read1 after filtering: total reads: 30223 total bases: 2527723 Q20 bases: 2467046(97.5995%) Q30 bases: 2389288(94.5233%) Filtering result: reads passed filter: 30223 reads failed due to low quality: 159 reads failed due to too many N: 0 reads failed due to too short: 19618 reads with adapter trimmed: 338 bases trimmed due to adapters: 2571 reads with polyX in 3' end: 22695 bases trimmed in polyX tail: 1341198 Duplication rate (may be overestimated since this is SE data): 17.534% JSON report: SRX7656982.fastp.json HTML report: SRX7656982.fastp.html fastp --in1 SRX7656982.fastq.gz --out1 SRX7656982.fastp.fastq.gz --thread 6 --json SRX7656982.fastp.json --html SRX7656982.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds