WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4158496 Q20 bases: 4066974(97.7992%) Q30 bases: 3943383(94.8271%) Read1 after filtering: total reads: 29641 total bases: 2434615 Q20 bases: 2374611(97.5354%) Q30 bases: 2300100(94.4749%) Filtering result: reads passed filter: 29641 reads failed due to low quality: 192 reads failed due to too many N: 0 reads failed due to too short: 20167 reads with adapter trimmed: 439 bases trimmed due to adapters: 3528 reads with polyX in 3' end: 23887 bases trimmed in polyX tail: 1193488 Duplication rate (may be overestimated since this is SE data): 20.928% JSON report: SRX7656981.fastp.json HTML report: SRX7656981.fastp.html fastp --in1 SRX7656981.fastq.gz --out1 SRX7656981.fastp.fastq.gz --thread 6 --json SRX7656981.fastp.json --html SRX7656981.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds