WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4201122 Q20 bases: 4113431(97.9127%) Q30 bases: 3991628(95.0134%) Read1 after filtering: total reads: 25370 total bases: 2065521 Q20 bases: 2014446(97.5273%) Q30 bases: 1950100(94.412%) Filtering result: reads passed filter: 25370 reads failed due to low quality: 202 reads failed due to too many N: 0 reads failed due to too short: 24428 reads with adapter trimmed: 408 bases trimmed due to adapters: 3225 reads with polyX in 3' end: 27658 bases trimmed in polyX tail: 1596008 Duplication rate (may be overestimated since this is SE data): 21.954% JSON report: SRX7656980.fastp.json HTML report: SRX7656980.fastp.html fastp --in1 SRX7656980.fastq.gz --out1 SRX7656980.fastp.fastq.gz --thread 6 --json SRX7656980.fastp.json --html SRX7656980.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds