WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4433604 Q20 bases: 4341976(97.9333%) Q30 bases: 4215235(95.0747%) Read1 after filtering: total reads: 30772 total bases: 2588156 Q20 bases: 2527028(97.6382%) Q30 bases: 2447765(94.5756%) Filtering result: reads passed filter: 30772 reads failed due to low quality: 140 reads failed due to too many N: 0 reads failed due to too short: 19088 reads with adapter trimmed: 341 bases trimmed due to adapters: 2612 reads with polyX in 3' end: 21863 bases trimmed in polyX tail: 1311179 Duplication rate (may be overestimated since this is SE data): 17.978% JSON report: SRX7656979.fastp.json HTML report: SRX7656979.fastp.html fastp --in1 SRX7656979.fastq.gz --out1 SRX7656979.fastp.fastq.gz --thread 6 --json SRX7656979.fastp.json --html SRX7656979.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds