WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4152743 Q20 bases: 4058033(97.7193%) Q30 bases: 3929848(94.6326%) Read1 after filtering: total reads: 28997 total bases: 2349494 Q20 bases: 2287681(97.3691%) Q30 bases: 2210809(94.0972%) Filtering result: reads passed filter: 28997 reads failed due to low quality: 162 reads failed due to too many N: 0 reads failed due to too short: 20841 reads with adapter trimmed: 595 bases trimmed due to adapters: 4706 reads with polyX in 3' end: 24127 bases trimmed in polyX tail: 1270803 Duplication rate (may be overestimated since this is SE data): 24.226% JSON report: SRX7656978.fastp.json HTML report: SRX7656978.fastp.html fastp --in1 SRX7656978.fastq.gz --out1 SRX7656978.fastp.fastq.gz --thread 6 --json SRX7656978.fastp.json --html SRX7656978.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds