WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4502694 Q20 bases: 4414424(98.0396%) Q30 bases: 4291660(95.3132%) Read1 after filtering: total reads: 32959 total bases: 2776716 Q20 bases: 2716011(97.8138%) Q30 bases: 2636830(94.9622%) Filtering result: reads passed filter: 32959 reads failed due to low quality: 138 reads failed due to too many N: 0 reads failed due to too short: 16903 reads with adapter trimmed: 251 bases trimmed due to adapters: 1900 reads with polyX in 3' end: 20959 bases trimmed in polyX tail: 1194660 Duplication rate (may be overestimated since this is SE data): 12.964% JSON report: SRX7656977.fastp.json HTML report: SRX7656977.fastp.html fastp --in1 SRX7656977.fastq.gz --out1 SRX7656977.fastp.fastq.gz --thread 6 --json SRX7656977.fastp.json --html SRX7656977.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds