WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4356040 Q20 bases: 4263048(97.8652%) Q30 bases: 4136710(94.9649%) Read1 after filtering: total reads: 29388 total bases: 2450007 Q20 bases: 2388455(97.4877%) Q30 bases: 2310567(94.3086%) Filtering result: reads passed filter: 29388 reads failed due to low quality: 163 reads failed due to too many N: 0 reads failed due to too short: 20449 reads with adapter trimmed: 398 bases trimmed due to adapters: 3078 reads with polyX in 3' end: 23109 bases trimmed in polyX tail: 1372953 Duplication rate (may be overestimated since this is SE data): 21.418% JSON report: SRX7656976.fastp.json HTML report: SRX7656976.fastp.html fastp --in1 SRX7656976.fastq.gz --out1 SRX7656976.fastp.fastq.gz --thread 6 --json SRX7656976.fastp.json --html SRX7656976.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds