WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4409081 Q20 bases: 4320919(98.0004%) Q30 bases: 4199414(95.2447%) Read1 after filtering: total reads: 30332 total bases: 2568001 Q20 bases: 2509440(97.7196%) Q30 bases: 2434801(94.8131%) Filtering result: reads passed filter: 30332 reads failed due to low quality: 175 reads failed due to too many N: 0 reads failed due to too short: 19493 reads with adapter trimmed: 320 bases trimmed due to adapters: 2505 reads with polyX in 3' end: 22206 bases trimmed in polyX tail: 1308608 Duplication rate (may be overestimated since this is SE data): 19.34% JSON report: SRX7656975.fastp.json HTML report: SRX7656975.fastp.html fastp --in1 SRX7656975.fastq.gz --out1 SRX7656975.fastp.fastq.gz --thread 6 --json SRX7656975.fastp.json --html SRX7656975.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds