WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4308413 Q20 bases: 4219215(97.9297%) Q30 bases: 4097293(95.0998%) Read1 after filtering: total reads: 28399 total bases: 2364322 Q20 bases: 2307075(97.5787%) Q30 bases: 2235417(94.5479%) Filtering result: reads passed filter: 28399 reads failed due to low quality: 196 reads failed due to too many N: 0 reads failed due to too short: 21405 reads with adapter trimmed: 376 bases trimmed due to adapters: 2948 reads with polyX in 3' end: 24284 bases trimmed in polyX tail: 1410383 Duplication rate (may be overestimated since this is SE data): 21.526% JSON report: SRX7656974.fastp.json HTML report: SRX7656974.fastp.html fastp --in1 SRX7656974.fastq.gz --out1 SRX7656974.fastp.fastq.gz --thread 6 --json SRX7656974.fastp.json --html SRX7656974.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds