WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4232430 Q20 bases: 4144339(97.9187%) Q30 bases: 4022741(95.0457%) Read1 after filtering: total reads: 23540 total bases: 1905141 Q20 bases: 1856004(97.4208%) Q30 bases: 1794538(94.1945%) Filtering result: reads passed filter: 23540 reads failed due to low quality: 160 reads failed due to too many N: 0 reads failed due to too short: 26300 reads with adapter trimmed: 425 bases trimmed due to adapters: 3473 reads with polyX in 3' end: 29216 bases trimmed in polyX tail: 1788787 Duplication rate (may be overestimated since this is SE data): 24.602% JSON report: SRX7656973.fastp.json HTML report: SRX7656973.fastp.html fastp --in1 SRX7656973.fastq.gz --out1 SRX7656973.fastp.fastq.gz --thread 6 --json SRX7656973.fastp.json --html SRX7656973.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds