WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4416454 Q20 bases: 4326481(97.9628%) Q30 bases: 4202710(95.1603%) Read1 after filtering: total reads: 31113 total bases: 2644406 Q20 bases: 2584201(97.7233%) Q30 bases: 2507099(94.8076%) Filtering result: reads passed filter: 31113 reads failed due to low quality: 179 reads failed due to too many N: 0 reads failed due to too short: 18708 reads with adapter trimmed: 366 bases trimmed due to adapters: 2894 reads with polyX in 3' end: 21148 bases trimmed in polyX tail: 1240442 Duplication rate (may be overestimated since this is SE data): 21.746% JSON report: SRX7656972.fastp.json HTML report: SRX7656972.fastp.html fastp --in1 SRX7656972.fastq.gz --out1 SRX7656972.fastp.fastq.gz --thread 6 --json SRX7656972.fastp.json --html SRX7656972.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds