WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4295032 Q20 bases: 4201934(97.8324%) Q30 bases: 4076473(94.9114%) Read1 after filtering: total reads: 33993 total bases: 2799271 Q20 bases: 2732295(97.6074%) Q30 bases: 2647025(94.5612%) Filtering result: reads passed filter: 33993 reads failed due to low quality: 182 reads failed due to too many N: 0 reads failed due to too short: 15825 reads with adapter trimmed: 320 bases trimmed due to adapters: 2538 reads with polyX in 3' end: 20597 bases trimmed in polyX tail: 957093 Duplication rate (may be overestimated since this is SE data): 17.228% JSON report: SRX7656970.fastp.json HTML report: SRX7656970.fastp.html fastp --in1 SRX7656970.fastq.gz --out1 SRX7656970.fastp.fastq.gz --thread 6 --json SRX7656970.fastp.json --html SRX7656970.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds