WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4152179 Q20 bases: 4059846(97.7763%) Q30 bases: 3935225(94.7749%) Read1 after filtering: total reads: 25205 total bases: 2022806 Q20 bases: 1967432(97.2625%) Q30 bases: 1899155(93.8872%) Filtering result: reads passed filter: 25205 reads failed due to low quality: 198 reads failed due to too many N: 0 reads failed due to too short: 24597 reads with adapter trimmed: 615 bases trimmed due to adapters: 4907 reads with polyX in 3' end: 27244 bases trimmed in polyX tail: 1587093 Duplication rate (may be overestimated since this is SE data): 25.156% JSON report: SRX7656969.fastp.json HTML report: SRX7656969.fastp.html fastp --in1 SRX7656969.fastq.gz --out1 SRX7656969.fastp.fastq.gz --thread 6 --json SRX7656969.fastp.json --html SRX7656969.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds