WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4034932 Q20 bases: 3947178(97.8251%) Q30 bases: 3828274(94.8783%) Read1 after filtering: total reads: 24276 total bases: 1930202 Q20 bases: 1878321(97.3121%) Q30 bases: 1815481(94.0565%) Filtering result: reads passed filter: 24276 reads failed due to low quality: 179 reads failed due to too many N: 0 reads failed due to too short: 25545 reads with adapter trimmed: 586 bases trimmed due to adapters: 4694 reads with polyX in 3' end: 28686 bases trimmed in polyX tail: 1571308 Duplication rate (may be overestimated since this is SE data): 27.598% JSON report: SRX7656968.fastp.json HTML report: SRX7656968.fastp.html fastp --in1 SRX7656968.fastq.gz --out1 SRX7656968.fastp.fastq.gz --thread 6 --json SRX7656968.fastp.json --html SRX7656968.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds