WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4240131 Q20 bases: 4148992(97.8506%) Q30 bases: 4024627(94.9175%) Read1 after filtering: total reads: 24482 total bases: 1959094 Q20 bases: 1907526(97.3678%) Q30 bases: 1843931(94.1216%) Filtering result: reads passed filter: 24482 reads failed due to low quality: 218 reads failed due to too many N: 0 reads failed due to too short: 25300 reads with adapter trimmed: 461 bases trimmed due to adapters: 3698 reads with polyX in 3' end: 28823 bases trimmed in polyX tail: 1737934 Duplication rate (may be overestimated since this is SE data): 21.578% JSON report: SRX7656967.fastp.json HTML report: SRX7656967.fastp.html fastp --in1 SRX7656967.fastq.gz --out1 SRX7656967.fastp.fastq.gz --thread 6 --json SRX7656967.fastp.json --html SRX7656967.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds