WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4134818 Q20 bases: 4044606(97.8182%) Q30 bases: 3921621(94.8439%) Read1 after filtering: total reads: 24513 total bases: 1940261 Q20 bases: 1888714(97.3433%) Q30 bases: 1825631(94.092%) Filtering result: reads passed filter: 24513 reads failed due to low quality: 192 reads failed due to too many N: 0 reads failed due to too short: 25295 reads with adapter trimmed: 550 bases trimmed due to adapters: 4450 reads with polyX in 3' end: 28837 bases trimmed in polyX tail: 1651983 Duplication rate (may be overestimated since this is SE data): 23.918% JSON report: SRX7656965.fastp.json HTML report: SRX7656965.fastp.html fastp --in1 SRX7656965.fastq.gz --out1 SRX7656965.fastp.fastq.gz --thread 6 --json SRX7656965.fastp.json --html SRX7656965.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 2 seconds