WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 3915282 Q20 bases: 3828969(97.7955%) Q30 bases: 3712648(94.8245%) Read1 after filtering: total reads: 21298 total bases: 1649742 Q20 bases: 1602133(97.1142%) Q30 bases: 1545313(93.67%) Filtering result: reads passed filter: 21298 reads failed due to low quality: 193 reads failed due to too many N: 0 reads failed due to too short: 28509 reads with adapter trimmed: 669 bases trimmed due to adapters: 5378 reads with polyX in 3' end: 31142 bases trimmed in polyX tail: 1720022 Duplication rate (may be overestimated since this is SE data): 31.662% JSON report: SRX7656963.fastp.json HTML report: SRX7656963.fastp.html fastp --in1 SRX7656963.fastq.gz --out1 SRX7656963.fastp.fastq.gz --thread 6 --json SRX7656963.fastp.json --html SRX7656963.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 2 seconds