WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4199843 Q20 bases: 4111118(97.8874%) Q30 bases: 3990323(95.0112%) Read1 after filtering: total reads: 26593 total bases: 2170494 Q20 bases: 2115593(97.4706%) Q30 bases: 2047485(94.3327%) Filtering result: reads passed filter: 26593 reads failed due to low quality: 197 reads failed due to too many N: 0 reads failed due to too short: 23210 reads with adapter trimmed: 431 bases trimmed due to adapters: 3408 reads with polyX in 3' end: 26259 bases trimmed in polyX tail: 1490674 Duplication rate (may be overestimated since this is SE data): 20.248% JSON report: SRX7656962.fastp.json HTML report: SRX7656962.fastp.html fastp --in1 SRX7656962.fastq.gz --out1 SRX7656962.fastp.fastq.gz --thread 6 --json SRX7656962.fastp.json --html SRX7656962.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds