WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4175353 Q20 bases: 4085508(97.8482%) Q30 bases: 3963173(94.9183%) Read1 after filtering: total reads: 27610 total bases: 2262618 Q20 bases: 2205600(97.48%) Q30 bases: 2134395(94.333%) Filtering result: reads passed filter: 27610 reads failed due to low quality: 141 reads failed due to too many N: 0 reads failed due to too short: 22249 reads with adapter trimmed: 462 bases trimmed due to adapters: 3676 reads with polyX in 3' end: 25374 bases trimmed in polyX tail: 1382072 Duplication rate (may be overestimated since this is SE data): 24.438% JSON report: SRX7656961.fastp.json HTML report: SRX7656961.fastp.html fastp --in1 SRX7656961.fastq.gz --out1 SRX7656961.fastp.fastq.gz --thread 6 --json SRX7656961.fastp.json --html SRX7656961.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds