WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4110515 Q20 bases: 4024457(97.9064%) Q30 bases: 3905867(95.0214%) Read1 after filtering: total reads: 23226 total bases: 1854283 Q20 bases: 1803731(97.2738%) Q30 bases: 1741614(93.9239%) Filtering result: reads passed filter: 23226 reads failed due to low quality: 153 reads failed due to too many N: 0 reads failed due to too short: 26621 reads with adapter trimmed: 562 bases trimmed due to adapters: 4449 reads with polyX in 3' end: 29161 bases trimmed in polyX tail: 1724718 Duplication rate (may be overestimated since this is SE data): 32.098% JSON report: SRX7656960.fastp.json HTML report: SRX7656960.fastp.html fastp --in1 SRX7656960.fastq.gz --out1 SRX7656960.fastp.fastq.gz --thread 6 --json SRX7656960.fastp.json --html SRX7656960.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds