WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4194071 Q20 bases: 4104092(97.8546%) Q30 bases: 3981127(94.9227%) Read1 after filtering: total reads: 26884 total bases: 2194744 Q20 bases: 2139462(97.4812%) Q30 bases: 2070589(94.3431%) Filtering result: reads passed filter: 26884 reads failed due to low quality: 185 reads failed due to too many N: 0 reads failed due to too short: 22931 reads with adapter trimmed: 451 bases trimmed due to adapters: 3569 reads with polyX in 3' end: 25928 bases trimmed in polyX tail: 1462397 Duplication rate (may be overestimated since this is SE data): 21.382% JSON report: SRX7656959.fastp.json HTML report: SRX7656959.fastp.html fastp --in1 SRX7656959.fastq.gz --out1 SRX7656959.fastp.fastq.gz --thread 6 --json SRX7656959.fastp.json --html SRX7656959.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds