WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4083781 Q20 bases: 3990761(97.7222%) Q30 bases: 3866695(94.6842%) Read1 after filtering: total reads: 27383 total bases: 2219554 Q20 bases: 2160570(97.3425%) Q30 bases: 2088190(94.0815%) Filtering result: reads passed filter: 27383 reads failed due to low quality: 147 reads failed due to too many N: 0 reads failed due to too short: 22470 reads with adapter trimmed: 583 bases trimmed due to adapters: 4648 reads with polyX in 3' end: 25552 bases trimmed in polyX tail: 1332881 Duplication rate (may be overestimated since this is SE data): 23.74% JSON report: SRX7656958.fastp.json HTML report: SRX7656958.fastp.html fastp --in1 SRX7656958.fastq.gz --out1 SRX7656958.fastp.fastq.gz --thread 6 --json SRX7656958.fastp.json --html SRX7656958.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds