WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4041684 Q20 bases: 3953984(97.8301%) Q30 bases: 3834473(94.8732%) Read1 after filtering: total reads: 24037 total bases: 1912054 Q20 bases: 1861554(97.3589%) Q30 bases: 1799591(94.1182%) Filtering result: reads passed filter: 24037 reads failed due to low quality: 201 reads failed due to too many N: 0 reads failed due to too short: 25762 reads with adapter trimmed: 578 bases trimmed due to adapters: 4700 reads with polyX in 3' end: 28756 bases trimmed in polyX tail: 1597117 Duplication rate (may be overestimated since this is SE data): 29.218% JSON report: SRX7656957.fastp.json HTML report: SRX7656957.fastp.html fastp --in1 SRX7656957.fastq.gz --out1 SRX7656957.fastp.fastq.gz --thread 6 --json SRX7656957.fastp.json --html SRX7656957.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds