WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 50000
total bases: 4308413
Q20 bases: 4219215(97.9297%)
Q30 bases: 4097293(95.0998%)

Read1 after filtering:
total reads: 27045
total bases: 2305244
Q20 bases: 2252510(97.7124%)
Q30 bases: 2185108(94.7886%)

Filtering result:
reads passed filter: 27045
reads failed due to low quality: 54
reads failed due to too many N: 0
reads failed due to too short: 22901
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 21.526%

JSON report: SRX7656974.fastp.json
HTML report: SRX7656974.fastp.html

fastp --in1 SRX7656974.fastq.gz --out1 SRX7656974.fastp.fastq.gz --thread 6 --json SRX7656974.fastp.json --html SRX7656974.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250407_RNAseq/adapter_fasta/polyA.fa 
fastp v0.23.4, time used: 2 seconds