WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 50000 total bases: 4232430 Q20 bases: 4144339(97.9187%) Q30 bases: 4022741(95.0457%) Read1 after filtering: total reads: 21976 total bases: 1834587 Q20 bases: 1790877(97.6174%) Q30 bases: 1734624(94.5512%) Filtering result: reads passed filter: 21976 reads failed due to low quality: 56 reads failed due to too many N: 0 reads failed due to too short: 27968 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 24.602% JSON report: SRX7656973.fastp.json HTML report: SRX7656973.fastp.html fastp --in1 SRX7656973.fastq.gz --out1 SRX7656973.fastp.fastq.gz --thread 6 --json SRX7656973.fastp.json --html SRX7656973.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250407_RNAseq/adapter_fasta/polyA.fa fastp v0.23.4, time used: 3 seconds