WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 50000 total bases: 4240131 Q20 bases: 4148992(97.8506%) Q30 bases: 4024627(94.9175%) Read1 after filtering: total reads: 22637 total bases: 1875845 Q20 bases: 1830889(97.6034%) Q30 bases: 1773531(94.5457%) Filtering result: reads passed filter: 22637 reads failed due to low quality: 58 reads failed due to too many N: 0 reads failed due to too short: 27305 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 21.578% JSON report: SRX7656967.fastp.json HTML report: SRX7656967.fastp.html fastp --in1 SRX7656967.fastq.gz --out1 SRX7656967.fastp.fastq.gz --thread 6 --json SRX7656967.fastp.json --html SRX7656967.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250407_RNAseq/adapter_fasta/polyA.fa fastp v0.23.4, time used: 2 seconds