WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 50000
total bases: 4175353
Q20 bases: 4085508(97.8482%)
Q30 bases: 3963173(94.9183%)

Read1 after filtering:
total reads: 25875
total bases: 2183608
Q20 bases: 2132474(97.6583%)
Q30 bases: 2066903(94.6554%)

Filtering result:
reads passed filter: 25875
reads failed due to low quality: 45
reads failed due to too many N: 0
reads failed due to too short: 24080
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 24.438%

JSON report: SRX7656961.fastp.json
HTML report: SRX7656961.fastp.html

fastp --in1 SRX7656961.fastq.gz --out1 SRX7656961.fastp.fastq.gz --thread 6 --json SRX7656961.fastp.json --html SRX7656961.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250407_RNAseq/adapter_fasta/polyA.fa 
fastp v0.23.4, time used: 2 seconds