WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 50000
total bases: 4502694
Q20 bases: 4414424(98.0396%)
Q30 bases: 4291660(95.3132%)

Read1 after filtering:
total reads: 31821
total bases: 2716984
Q20 bases: 2660695(97.9283%)
Q30 bases: 2585587(95.1639%)

Filtering result:
reads passed filter: 31821
reads failed due to low quality: 40
reads failed due to too many N: 0
reads failed due to too short: 18139
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 12.964%

JSON report: SRX7656977.fastp.json
HTML report: SRX7656977.fastp.html

fastp --in1 SRX7656977.fastq.gz --out1 SRX7656977.fastp.fastq.gz --thread 6 --json SRX7656977.fastp.json --html SRX7656977.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250407_RNAseq/adapter_fasta/3ILL-30_5ILL/polyA.fa 
fastp v0.23.4, time used: 5 seconds