SUMMARISING RUN PARAMETERS
==========================
Input filename: zr3644_7_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.9
Python version: could not detect
Number of cores used for trimming: 8
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Unable to auto-detect most prominent adapter from the first specified file (count Nextera: 0, count smallRNA: 0, count Illumina: 0)
Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Running FastQC on the data once trimming has completed
Output file will be GZIP compressed


This is cutadapt 4.9 with Python 3.12.8
Command line parameters: -j 8 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC zr3644_7_1.fastq.gz
Processing single-end reads on 8 cores ...
Finished in 298.685 s (3.662 µs/read; 16.39 M reads/minute).

=== Summary ===

Total reads processed:              81,572,749
Reads with adapters:                18,048,115 (22.1%)
Reads written (passing filters):    81,572,749 (100.0%)

Total basepairs processed: 8,628,118,547 bp
Quality-trimmed:               1,748,406 bp (0.0%)
Total written (filtered):  8,606,658,724 bp (99.8%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 18048115 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 47.9%
  C: 11.3%
  G: 6.0%
  T: 34.7%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	17331689	20393187.2	0	17331689
2	16811	5098296.8	0	16811
3	454139	1274574.2	0	454139
4	244435	318643.6	0	244435
5	893	79660.9	0	893
6	8	19915.2	0	8
7	5	4978.8	0	5
8	4	1244.7	0	4
9	3	311.2	0	0 3
10	67	77.8	1	0 67
11	60	19.4	1	0 60
12	1	4.9	1	0 1

RUN STATISTICS FOR INPUT FILE: zr3644_7_1.fastq.gz
=============================================
81572749 sequences processed in total