Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/CTRL_8C_26psu_1_S17_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/CTRL_8C_26psu_1_S17_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 27136662 Number of paired-end alignments with a unique best hit: 11526081 Mapping efficiency: 42.5% Sequence pairs with no alignments under any condition: 10347684 Sequence pairs did not map uniquely: 5262897 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2945792 ((converted) top strand) GA/CT/CT: 2846878 (complementary to (converted) top strand) GA/CT/GA: 2818369 (complementary to (converted) bottom strand) CT/GA/GA: 2915042 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 438442419 Total methylated C's in CpG context: 30947464 Total methylated C's in CHG context: 706793 Total methylated C's in CHH context: 3185725 Total methylated C's in Unknown context: 68873 Total unmethylated C's in CpG context: 8642835 Total unmethylated C's in CHG context: 102494522 Total unmethylated C's in CHH context: 292465080 Total unmethylated C's in Unknown context: 347968 C methylated in CpG context: 78.2% C methylated in CHG context: 0.7% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 16.5% Bismark completed in 0d 1h 52m 57s