Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_32psu_3_S7_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_32psu_3_S7_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 27987516 Number of paired-end alignments with a unique best hit: 11212522 Mapping efficiency: 40.1% Sequence pairs with no alignments under any condition: 10199611 Sequence pairs did not map uniquely: 6575383 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2902963 ((converted) top strand) GA/CT/CT: 2722127 (complementary to (converted) top strand) GA/CT/GA: 2702031 (complementary to (converted) bottom strand) CT/GA/GA: 2885401 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 399773049 Total methylated C's in CpG context: 31583077 Total methylated C's in CHG context: 642600 Total methylated C's in CHH context: 3468138 Total methylated C's in Unknown context: 76861 Total unmethylated C's in CpG context: 9290652 Total unmethylated C's in CHG context: 99801597 Total unmethylated C's in CHH context: 254986985 Total unmethylated C's in Unknown context: 320376 C methylated in CpG context: 77.3% C methylated in CHG context: 0.6% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 19.3% Bismark completed in 0d 1h 46m 33s