Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_3_S11_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_3_S11_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 33969202 Number of paired-end alignments with a unique best hit: 13773586 Mapping efficiency: 40.5% Sequence pairs with no alignments under any condition: 11692059 Sequence pairs did not map uniquely: 8503557 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3611755 ((converted) top strand) GA/CT/CT: 3296848 (complementary to (converted) top strand) GA/CT/GA: 3280388 (complementary to (converted) bottom strand) CT/GA/GA: 3584595 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 468577771 Total methylated C's in CpG context: 39768872 Total methylated C's in CHG context: 826962 Total methylated C's in CHH context: 4200228 Total methylated C's in Unknown context: 99326 Total unmethylated C's in CpG context: 10682234 Total unmethylated C's in CHG context: 119874383 Total unmethylated C's in CHH context: 293225092 Total unmethylated C's in Unknown context: 367257 C methylated in CpG context: 78.8% C methylated in CHG context: 0.7% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 21.3% Bismark completed in 0d 2h 7m 17s