Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_2_S10_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_2_S10_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 36473118 Number of paired-end alignments with a unique best hit: 15449082 Mapping efficiency: 42.4% Sequence pairs with no alignments under any condition: 13192483 Sequence pairs did not map uniquely: 7831553 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4041183 ((converted) top strand) GA/CT/CT: 3701149 (complementary to (converted) top strand) GA/CT/GA: 3683607 (complementary to (converted) bottom strand) CT/GA/GA: 4023143 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 577534224 Total methylated C's in CpG context: 44037673 Total methylated C's in CHG context: 968953 Total methylated C's in CHH context: 4564857 Total methylated C's in Unknown context: 101806 Total unmethylated C's in CpG context: 12421237 Total unmethylated C's in CHG context: 142459334 Total unmethylated C's in CHH context: 373082170 Total unmethylated C's in Unknown context: 450657 C methylated in CpG context: 78.0% C methylated in CHG context: 0.7% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 18.4% Bismark completed in 0d 2h 22m 53s