Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_1_S9_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/8C_26psu_1_S9_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 35922181 Number of paired-end alignments with a unique best hit: 14030303 Mapping efficiency: 39.1% Sequence pairs with no alignments under any condition: 14894341 Sequence pairs did not map uniquely: 6997537 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3541345 ((converted) top strand) GA/CT/CT: 3499850 (complementary to (converted) top strand) GA/CT/GA: 3476574 (complementary to (converted) bottom strand) CT/GA/GA: 3512533 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 538202447 Total methylated C's in CpG context: 40951438 Total methylated C's in CHG context: 994353 Total methylated C's in CHH context: 4739798 Total methylated C's in Unknown context: 110622 Total unmethylated C's in CpG context: 11233486 Total unmethylated C's in CHG context: 133217931 Total unmethylated C's in CHH context: 347065441 Total unmethylated C's in Unknown context: 464809 C methylated in CpG context: 78.5% C methylated in CHG context: 0.7% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 19.2% Bismark completed in 0d 2h 20m 47s