Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_32psu_4_S4_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31149160 Number of paired-end alignments with a unique best hit: 12468000 Mapping efficiency: 40.0% Sequence pairs with no alignments under any condition: 13843507 Sequence pairs did not map uniquely: 4837653 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3128634 ((converted) top strand) GA/CT/CT: 3122325 (complementary to (converted) top strand) GA/CT/GA: 3105836 (complementary to (converted) bottom strand) CT/GA/GA: 3111205 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 513155617 Total methylated C's in CpG context: 36287531 Total methylated C's in CHG context: 829946 Total methylated C's in CHH context: 3691013 Total methylated C's in Unknown context: 103298 Total unmethylated C's in CpG context: 10429257 Total unmethylated C's in CHG context: 121877256 Total unmethylated C's in CHH context: 340040614 Total unmethylated C's in Unknown context: 446750 C methylated in CpG context: 77.7% C methylated in CHG context: 0.7% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 18.8% Bismark completed in 0d 1h 59m 58s