Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_26psu_4_S16_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_26psu_4_S16_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 34091207 Number of paired-end alignments with a unique best hit: 13426810 Mapping efficiency: 39.4% Sequence pairs with no alignments under any condition: 13479526 Sequence pairs did not map uniquely: 7184871 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3317334 ((converted) top strand) GA/CT/CT: 3422254 (complementary to (converted) top strand) GA/CT/GA: 3395707 (complementary to (converted) bottom strand) CT/GA/GA: 3291515 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 493382628 Total methylated C's in CpG context: 38275997 Total methylated C's in CHG context: 843559 Total methylated C's in CHH context: 4976221 Total methylated C's in Unknown context: 89481 Total unmethylated C's in CpG context: 10435235 Total unmethylated C's in CHG context: 122258415 Total unmethylated C's in CHH context: 316593201 Total unmethylated C's in Unknown context: 399130 C methylated in CpG context: 78.6% C methylated in CHG context: 0.7% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 18.3% Bismark completed in 0d 2h 14m 46s