Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_26psu_3_S15_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/20200612/16C_26psu_3_S15_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/2022/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 28985030 Number of paired-end alignments with a unique best hit: 11459125 Mapping efficiency: 39.5% Sequence pairs with no alignments under any condition: 11366010 Sequence pairs did not map uniquely: 6159895 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2781835 ((converted) top strand) GA/CT/CT: 2982966 (complementary to (converted) top strand) GA/CT/GA: 2947439 (complementary to (converted) bottom strand) CT/GA/GA: 2746885 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 427554162 Total methylated C's in CpG context: 30606235 Total methylated C's in CHG context: 817673 Total methylated C's in CHH context: 7811756 Total methylated C's in Unknown context: 72450 Total unmethylated C's in CpG context: 8121667 Total unmethylated C's in CHG context: 108185552 Total unmethylated C's in CHH context: 272011279 Total unmethylated C's in Unknown context: 338123 C methylated in CpG context: 79.0% C methylated in CHG context: 0.8% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 17.6% Bismark completed in 0d 2h 8m 15s