===================== Where are my files? ===================== By default, the nf-core/rnaseq pipeline does not save large intermediate files to the results directory. This is to try to conserve disk space. These files can be found in the pipeline `work` directory if needed. Alternatively, re-run the pipeline using `-resume` in addition to one of the below command-line options and they will be copied into the results directory: `--saveAlignedIntermediates` The final BAM files created after the Picard MarkDuplicates step are always saved and can be found in the `markDuplicates/` folder. Specify this flag to also copy out BAM files from STAR / HISAT2 alignment and sorting steps. `--saveTrimmed` Specify to save trimmed FastQ files to the results directory. `--saveReference` Save any downloaded or generated reference genome files to your results folder. These can then be used for future pipeline runs, reducing processing times. ----------------------------------- Setting defaults in a config file ----------------------------------- If you would always like these files to be saved without having to specify this on the command line, you can save the following to your personal configuration file (eg. `~/.nextflow/config`): params.saveReference = true params.saveTrimmed = true params.saveAlignedIntermediates = true For more help, see the following documentation: https://github.com/nf-core/rnaseq/blob/master/docs/usage.md https://www.nextflow.io/docs/latest/getstarted.html https://www.nextflow.io/docs/latest/config.html