HISAT2 summary stats: Total pairs: 27074146 Aligned concordantly or discordantly 0 time: 3574511 (13.20%) Aligned concordantly 1 time: 21943193 (81.05%) Aligned concordantly >1 times: 1556442 (5.75%) Aligned discordantly 1 time: 0 (0.00%) Total unpaired reads: 7149022 Aligned 0 time: 7149022 (100.00%) Aligned 1 time: 0 (0.00%) Aligned >1 times: 0 (0.00%) Overall alignment rate: 86.80%