HISAT2 summary stats: Total pairs: 20478570 Aligned concordantly or discordantly 0 time: 2638665 (12.89%) Aligned concordantly 1 time: 16779088 (81.93%) Aligned concordantly >1 times: 1060817 (5.18%) Aligned discordantly 1 time: 0 (0.00%) Total unpaired reads: 5277330 Aligned 0 time: 5277330 (100.00%) Aligned 1 time: 0 (0.00%) Aligned >1 times: 0 (0.00%) Overall alignment rate: 87.11%