HISAT2 summary stats: Total pairs: 25486439 Aligned concordantly or discordantly 0 time: 3264479 (12.81%) Aligned concordantly 1 time: 20611936 (80.87%) Aligned concordantly >1 times: 1610024 (6.32%) Aligned discordantly 1 time: 0 (0.00%) Total unpaired reads: 6528958 Aligned 0 time: 6528958 (100.00%) Aligned 1 time: 0 (0.00%) Aligned >1 times: 0 (0.00%) Overall alignment rate: 87.19%