Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/CTRL_16C_26psu_2_S21_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/CTRL_16C_26psu_2_S21_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 26574551 Number of paired-end alignments with a unique best hit: 9879876 Mapping efficiency: 37.2% Sequence pairs with no alignments under any condition: 11156490 Sequence pairs did not map uniquely: 5538185 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2640640 ((converted) top strand) GA/CT/CT: 2271291 (complementary to (converted) top strand) GA/CT/GA: 2297009 (complementary to (converted) bottom strand) CT/GA/GA: 2670934 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 338084645 Total methylated C's in CpG context: 30235769 Total methylated C's in CHG context: 746460 Total methylated C's in CHH context: 3093410 Total methylated C's in Unknown context: 97271 Total unmethylated C's in CpG context: 8154570 Total unmethylated C's in CHG context: 86382856 Total unmethylated C's in CHH context: 209471580 Total unmethylated C's in Unknown context: 341339 C methylated in CpG context: 78.8% C methylated in CHG context: 0.9% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 22.2% Bismark completed in 0d 1h 21m 59s