Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/8C_32psu_4_S8_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/8C_32psu_4_S8_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 29162017 Number of paired-end alignments with a unique best hit: 11254588 Mapping efficiency: 38.6% Sequence pairs with no alignments under any condition: 13313520 Sequence pairs did not map uniquely: 4593909 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2826840 ((converted) top strand) GA/CT/CT: 2779722 (complementary to (converted) top strand) GA/CT/GA: 2801990 (complementary to (converted) bottom strand) CT/GA/GA: 2846036 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 454276785 Total methylated C's in CpG context: 33372009 Total methylated C's in CHG context: 717954 Total methylated C's in CHH context: 2811980 Total methylated C's in Unknown context: 109227 Total unmethylated C's in CpG context: 10689210 Total unmethylated C's in CHG context: 108381935 Total unmethylated C's in CHH context: 298303697 Total unmethylated C's in Unknown context: 446724 C methylated in CpG context: 75.7% C methylated in CHG context: 0.7% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 19.6% Bismark completed in 0d 1h 40m 29s