Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/8C_26psu_3_S11_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/8C_26psu_3_S11_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 33969202 Number of paired-end alignments with a unique best hit: 13914221 Mapping efficiency: 41.0% Sequence pairs with no alignments under any condition: 12338561 Sequence pairs did not map uniquely: 7716420 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3618523 ((converted) top strand) GA/CT/CT: 3305647 (complementary to (converted) top strand) GA/CT/GA: 3336933 (complementary to (converted) bottom strand) CT/GA/GA: 3653117 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 471392414 Total methylated C's in CpG context: 39751927 Total methylated C's in CHG context: 851839 Total methylated C's in CHH context: 4403542 Total methylated C's in Unknown context: 99061 Total unmethylated C's in CpG context: 11144219 Total unmethylated C's in CHG context: 120441942 Total unmethylated C's in CHH context: 294798945 Total unmethylated C's in Unknown context: 382523 C methylated in CpG context: 78.1% C methylated in CHG context: 0.7% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 20.6% Bismark completed in 0d 1h 55m 42s