Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/8C_26psu_2_S10_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/8C_26psu_2_S10_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 36473118 Number of paired-end alignments with a unique best hit: 15526271 Mapping efficiency: 42.6% Sequence pairs with no alignments under any condition: 13952498 Sequence pairs did not map uniquely: 6994349 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4032212 ((converted) top strand) GA/CT/CT: 3691926 (complementary to (converted) top strand) GA/CT/GA: 3726392 (complementary to (converted) bottom strand) CT/GA/GA: 4075740 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 577960714 Total methylated C's in CpG context: 43750881 Total methylated C's in CHG context: 989876 Total methylated C's in CHH context: 4730506 Total methylated C's in Unknown context: 101714 Total unmethylated C's in CpG context: 12919516 Total unmethylated C's in CHG context: 142390693 Total unmethylated C's in CHH context: 373179242 Total unmethylated C's in Unknown context: 464631 C methylated in CpG context: 77.2% C methylated in CHG context: 0.7% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 18.0% Bismark completed in 0d 2h 10m 0s